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Subject Area

Hematology

Article Type

Original Study

Abstract

Objectives To study the expression of the immunophenotypic markers CD43 and CD180 in chronic lymphocytic leukemia (CLL) and to investigate their utility to discriminate between CLL and non-CLL cases. Background The established diagnostic criteria for CLL depend on morphology and immunophenotypic pattern. CLL usually co‐express CD5 and CD23 with FMC7 and CD79b are usually negative or weakly expressed. However, some CLL cases may have an atypical immunophenotype (for example, CD5‐ or CD23‐, FMC7+ or CD79b bright +), additional markers are needed for such cases to achieve a definitive diagnosis and to rule out other lymphomas. Methods Flow cytometry technique was used to analyze the expression of CD43, CD180 and other immunophenotypic markers of the Moreau Score. Peripheral blood or bone marrow samples were withdrawn from 53 newly diagnosed patients with mature B-cell neoplasms. Results CD43 was positively expressed in all CLL cases while intracellular expression (IC) of CD180 was found to be positive in 89.5% of CLL cases. Data analysis demonstrated that the percentage of CD43 expression was significantly higher in CLL in comparison with non-CLL group (P

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