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Subject Area

Clinical Pathology

Article Type

Original Study

Abstract

Objective To investigate the in vitro quality of room temperature stored apheresis platelet concentrates (PCs) with and without adding a platelet additive solution (PAS) and to evaluate its therapeutic utility in oncology patients. Background Several studies have highlighted using PAS as a way to improve the storage conditions of PCs. PAS is a synthetic media used in the place of plasma for platelet (PLT) storage. It is used to support metabolism, provide buffering capacity, and to protect PLTs from the storage lesion. So, it can be used to extend shelf life of PCs. To date, its application in Egyptian blood banks has not been increased. Patients and methods Twenty apheresis PCs were collected and stored at room temperature and divided into two groups; A1 group suspended in 100% plasma and tested on days 0 and 5 and A2 group suspended in 65% SSP + PAS and tested on days 0, 5, 7, and 10 of storage for PLT count, mean platelet volume, pH, partial pressure of oxygen, partial pressure of carbon dioxide, bicarbonate level, swirling, bacterial examination, glucose, lactate, lactate dehydrogenase, annexin expression, and PLT aggregation. SSP + PCs were transfused to oncology patients against plasma PCs and the corrected count increment (CCI) was calculated. Results SSP + PCs were comparable to plasma PCs up to day 7 of storage regarding all studied parameters when compared with day 5 of group A1; 7 days SSP + PCs showed a CCI comparable to plasma PCs. Conclusion We reported a maintained PLT function and metabolism up to 7 days of storage of apheresis PCs in 65% SSP + with a CCI comparable to plasma PCs.

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