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Subject Area

Microbiology

Article Type

Original Study

Abstract

Background Carbapenemase-producing carbapenem-resistant Enterobacteriaceae are a significant problem worldwide that causes life-threatening infections. Objectives To evaluate the performance of the phenotypic method [modified carbapenem-inactivation method (mCIM) and EDTA-modified carbapenem inactivation method (eCIM)] tests to detect carbapenemase production compared with the genotypic method (real-time PCR) in Escherichia coli and Klebsiella species strains that were isolated from patients in the Menoufia University Hospitals. Methods E. coli and Klebsiella species strains were isolated from patients in different departments of the Menoufia University Hospitals. The isolated strains were tested by the phenotypic method (mCIM and eCIM) tests to detect carbapenemase production, and screened by the real-time PCR for the presence of the carbapenemase gene (blaOXA-48). Results This study showed that the percent of carbapenem-resistant strains that were positive for mCIM was 72.3%, while 27.7% were negative for the test. Regarding eCIM, 46.2% of the carbapenem-resistant E. coli and Klebsiella species-strain isolates were positive, while 53.8% were negative for the test. The use of mCIM test combined with eCIM (mCIM/eCIM) showed a positivity of 46.2%. The percent of the carbapenem-resistant E. coli and Klebsiella species strains that harbored the blaOXA-48 gene was 60%, while 40% were negative for the gene. About 84.6% of the carbapenem-resistant strains that harbored the blaOXA-48 gene were positive for the mCIM test. Conclusion There was a reasonable agreement between the phenotypic method and molecular method for detection of carbapenemases; however, further study on a large number of samples is recommended.

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