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Subject Area

Dermatology

Article Type

Original Study

Abstract

Objective The aim of this study was to assess the ability of mesenchymal stem cells (MSCs) to differentiate into chondrocytes in vitro. Background MSCs with their ability of self-renewal and multilineage differentiation can differentiate into several types of cells including chondrocytes. It is reported that adult cartilage lacks the ability to repair itself, making articular cartilage a very attractive target for regenerative medicine therapy. Materials and methods This study was conducted at Clinical Pathology, Gynecology and Obstetrics Departments, Menoufia University Hospital, from December 2016 to May 2018, and included a collection of 30 umbilical cord samples under complete aseptic conditions, after obtaining Menoufia ethical committee approval. Samples were processed with the explant method. MSCs were separated using plastic adherence. MSCs were subcultured in a petri dish 35 mm2 then treated with chondrocytes' differentiation media. Results Identification of isolated MSCs was carried out by morphology and flow cytometry. MSCs were positive to flow cytometric markers CD44 and CD73 and negative for CD34 with a highly statistically significant difference P value of less than 0.001 in comparison with differentiated chondrogenic cells. MSCs were able to differentiate into chondrocytes after adding of chondrogenic differentiation media. Chondrocytes could be identified by morphology and immunocytochemistry method. Chondrogenic cells showed negative flow cytometric markers CD44 and CD73. Conclusion This study showed the ability of MSCs to differentiate into chondrocytes after the adding of chondrogenic differentiation media in vitro as a future therapy for skeletal diseases.

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