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Subject Area

Pediatrics

Article Type

Original Study

Abstract

Objective The objective of this study was to evaluate CD62L expression in chronic lymphocytic leukemia (CLL) and its effect on survival of malignant B cells. Background CLL is the most common adult leukemia and often presents as an early-stage disease. It could be stable for many years with no or minimal intervention. CD62L is one of the selectin family of adhesion molecules that plays an important role in the trafficking/homing of lymphocytes to the lymph node. Patients and methods This study was conducted on 35 patients with CLL and 15 age-matched and sex-matched healthy individuals as a control group. All patients were subjected to full history taking, clinical examination, and laboratory investigations. CD62L/CD19 expression on lymphocytes was measured for all the study participants using flow cytometry technique before and after cell culture. Results CD62L/CD19 expression increased minimally in controls at day 7 compared with day 0 (mean ± SD = 33.9 ± 5.3 and 18.03 ± 3.4, respectively) (P < 0.001), whereas the diseased group showed marked elevation at day 7 than day 0 (mean ± SD = 90.7 ± 6.4 and 36.9 ± 17.2, respectively) (P < 0.001). CD62L/CD19 expression in patients was higher than the controls at day 0 (mean ± SD = 36.9 ± 17.2 and 18.03 ± 3.4, respectively) and showed increase of approximately threefolds at day 7 (mean ± SD: 90.7 ± 6.4 and 33.9 ± 5.3, respectively) (P < 0.001). Conclusion There is high expression of CD62L on lymphocytic cells in patients with CLL associated with increased survival of malignant B cells.

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