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Subject Area

Pediatrics

Article Type

Case Report

Abstract

Objective The aim of this study was to assess the level of circulating endothelial cells (CECs) in women with preeclampsia (PE) in comparison with normotensive healthy pregnant controls. Background PE is a medical disorder that may affect pregnant women after 20th week of gestation, and in this condition, the patients experience new-onset hypertension and new-onset proteinuria or organ dysfunctional. There is placental ischemia with systemic maternal endothelium dysfunction. CECs are mature cells that shed from the endothelium in various diseases associated with vessel damage, and they are characterized as being CD146+ and CD45− cells. Patients and methods The current study included 30 patients with preeclampsia and 30 normotensive pregnant women as the control group. All the participants attended Department of Obstetrics and Gynecology, Faculty of Medicine, Menoufia University Hospitals. They were subjected to collection of demographic data (age and length of gestation), general clinical examination, in addition to undergoing some routine laboratory investigations. Flow cytometry was applied for quantification of CECs using both mouse antihuman fluorescein isothiocyanate-conjugated CD146 antibody and mouse antihuman phycoerythrin conjugated CD45 antibody. Results The CEC counts were significantly (P < 0.001) lower in women with PE (4026.67 ± 1464.5/5 × 106 peripheral mononuclear cells) in comparison with the healthy pregnant ones (9281.33 ± 1764.06/5 × 106 peripheral mononuclear cells). Additionally, CD146 expression strength (mean) was significantly (P < 0.001) higher in healthy pregnant women (93.81 ± 27.57) compared with that of those with PE (43.51 ± 14.47). Conclusion The number of CECs decreased significantly in patients with PE in comparison with normotensive healthy pregnant women.

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