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Subject Area

Pediatrics

Article Type

Original Study

Abstract

Objectives The aim of the present work is to study glutathione S-transferase (GST) genetic variants in patients with type 1 diabetes and its relation to its complications. Background Persistent oxidative stress is one of the several factors that participate in the pathogenesis of type 1 diabetes mellitus. GSTs are a family of antioxidant enzymes that exert important antioxidant roles in the elimination of reactive oxygen species. Patients and methods Forty type 1diabetes mellitus patients were compared with 20 healthy volunteers. GSTM1 and GSTT1 genotypes were screened using PCR and gel electrophoresis. Results The percentage of GSTT1-1 present genotype was 77.5% and GSTT1-0 null genotype was 22.5 versus 100% of the GSTT1-1 present genotype and 0% of the GSTT1-0 null genotype in control. This difference was found to be statistically significant with a P value = 0.023. There was significant positive association between GSTT1-0 null genotype and mean glycosylated hemoglobin in diabetic groups. Among the complicated cases which presented by peripheral numbness and nephropathy, GSTT1-0 null genotype showed a statistically significant association with P value = 0.007. Conclusion The GSTT1genotype may involve in the pathogenesis of type 1 diabetes mellitus and susceptibility to complication.

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