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Article Type

Original Study

Abstract

Objective The aim of this study was to study the serum level of surfactant protein-D (SP-D) and its genetic polymorphisms in chronic obstructive pulmonary disease (COPD) patients and its relation with severity. Background SP-D plays a role in COPD progression. It contributed toward the inflammatory response in the lungs. Single nucleotide polymorphisms of the surfactant protein-D gene (SFPTD) are believed to be associated with the risk of COPD. Patients and methods A case–control study was carried out on 76 individuals: group I included 50 patients with COPD and group II included 26 age-matched and sex-matched apparently healthy individuals. All participants were subjected to an assessment of history, clinical examination, BMI estimation, spirometry, serum SP-D by enzyme-linked immunosorbent assay, and genotyping of single nucleotide polymorphisms of SFPTD gene (rs721917, rs3088308) using the TaqMan allelic discrimination assay by real time PCR. Results Serum SP-D was significantly higher in patients than controls. The CT genotype of rs721719 was significantly more prevalent in patients. The T allele of both rs3088308 and rs721917 and smoking index were associated with increased SP-D levels. The T allele of rs721917 was associated with the risk of COPD. There was a significant difference between different genotypes of rs721917 in forced expiratory volume in 1 s (FEV1), forced volume capacity (FVC), FEV1/FVC, and serum SP-D. Conclusion SP-D can be used as a biomarker of the severity of COPD. The T allele of rs721917 might be a susceptible gene for COPD. The T allele of rs3088308 was associated with elevated serum SP-D, but not with COPD.

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