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Article Type

Original Study

Abstract

Objective The aim of the study was to determine the role of CD64 expression as a neutrophil surface marker in the early diagnosis of neonatal sepsis. Background Neonatal sepsis is a life-threatening disease with an incidence of 1-10 per 1000 live births and a mortality rate of 15-50%. The clinical signs are nonspecific and indistinguishable from those caused by a variety of neonatal noninfectious disorders. Patients and methods The studied population comprised 62 neonates with gestational ages of 26-41 weeks who were suspected to have sepsis (poor suckling, fever, vomiting, diarrhea, pallor, tachycardia, and others) in the first 15 days of life, and 18 healthy age and sex-matched neonates. Complete blood count analysis, C-reactive protein determination, blood culture, and flow cytometric analysis of CD64 expression on the neutrophil surface were carried out. Results CD64 expression was significantly higher in the group with sepsis than in the control groups (P < 0.001). Sensitivity and specificity of CD64 were 95 and 98.3%, respectively. The negative and positive predictive values of CD64 for identifying sepsis were 95 and 98.3%, respectively. Conclusion A change in cell surface expression of CD64 on peripheral blood neutrophils may be considered a sensitive marker for the detection of neonatal sepsis if used in combination with other laboratory parameters.

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