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Subject Area

Biochemistry

Article Type

Original Study

Abstract

Objectives: To evaluate serum level of TNFα and its gene polymorphism association with hepatocellular carcinoma

Background: HCC is one of the commonest fatal tumors worldwide, so it is a major international public health and considered the third leading cause of cancer death in the world.HCC is the second and sixth most prevalent cancer in men and women, respectively in Egypt. TNFα is a pleiotropic cytokine mainly produced by monocytes and macrophages. It has been linked to a number of physiological and pathological disorders. TNFα serum levels and its gene polymorphism have been shown to be significantly elevated in patients with various liver diseases.

Methods: This study included 105 participants: 40 HCC patients, 30 cirrhotic patients with no evidence of HCC presented to the Hepatology Department, National Liver Institute, Menoufia University, and 35 age and sex matched healthy controls. TNF serum levels were measured using ELISA, and its gene polymorphism (rs1799964) was determined by PCR.

Results: Our study found a significant statistical difference between HCC patients and control group, as well as between cirrhotic patients and control group, but no significant difference between HCC patients and cirrhotic patients as regards TNF polymorphism (rs1799964). Also we found a significant statistical difference in TNFserum levels between HCC patients and control group, cirrhotic patients and control group, and HCC patients and cirrhotic patients.

Conclusion: Serum levels of TNFα and its gene polymorphism (rs1799964) may be associated with an increased risk of HCC development.

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